rabbit antibody against s100a10  (Novus Biologicals)

Journal: Frontiers in Genetics

Article Title: KCNMB2-AS1 Promotes Bladder Cancer Progression Through Sponging miR-374a-3p to Upregulate S100A10

doi: 10.3389/fgene.2021.655569

Figure Lengend Snippet: miR-374a-3p targeted S100A10 in BC. (A) TargetScan predicted that S100A10 had a binding site for miR-374a-3p. (B) Dual luciferase report assay validated the interactions among miR-374a-3p and S100A10 in BC cells. (C,D) S100A10 expression was inhibited by miR-374a-3p mimics. (E,F) miR-374a-3p inhibitor reversed the expression of S100A10 inhibited by sh-KCNMB2-AS1. (G,H) Pearson’s correlation analysis was conducted to evaluate the correlation of S100A10 and miR-374a-3p or KCNMB2-AS1 in BC tissues. Data were showed as mean ± standard deviation. The experiment was repeated 3 times. * P < 0.05.

Article Snippet: After being blocked using 5% non-fat milk for 1 h at room temperature, the primary rabbit anti-human antibodies against S100A10 (ab76472, Abcam) and GAPDH (ab9485, Abcam) were supplemented overnight at 4°C.

Techniques: Binding Assay, Luciferase, Expressing, Standard Deviation

Journal: Frontiers in Genetics

Article Title: KCNMB2-AS1 Promotes Bladder Cancer Progression Through Sponging miR-374a-3p to Upregulate S100A10

doi: 10.3389/fgene.2021.655569

Figure Lengend Snippet: KCNMB2-AS1 promoted BC progression through miR-374a-3p/S100A10 axis. T24 cells were transfected with sh-NC + inhibitor NC, sh-KCNMB2-AS1 + inhibitor NC or miR-374a-3p inhibitor + sh-KCNMB2-AS1. (A,B) CCK8 and colony formation assay were performed. (C) Migration and invasion assay was performed. T24 cells were transfected with sh-NC + Vector, sh-KCNMB2-AS1 + Vector, sh-KCNMB2-AS1 + (overexpression, OE) OES100A10. (D,E) CCK8 and colony formation assay was performed. (F) Migration and invasion assay was performed. Data were shown as mean ± standard deviation. The experiment was repeated three times. * P < 0.05.

Article Snippet: After being blocked using 5% non-fat milk for 1 h at room temperature, the primary rabbit anti-human antibodies against S100A10 (ab76472, Abcam) and GAPDH (ab9485, Abcam) were supplemented overnight at 4°C.

Techniques: Transfection, Colony Assay, Migration, Invasion Assay, Plasmid Preparation, Over Expression, Standard Deviation

Journal: Frontiers in Genetics

Article Title: KCNMB2-AS1 Promotes Bladder Cancer Progression Through Sponging miR-374a-3p to Upregulate S100A10

doi: 10.3389/fgene.2021.655569

Figure Lengend Snippet: KCNMB2-AS1 knockdown inhibited BC cell growth in vivo. T24 cells transfected with sh-KCNMB2-AS1 or sh-NC were subcutaneously injected into nude mice to establish xenograft models. (A) Representative images of tumors. (B,C) The volume and weight of tumor xenografts. (D) Immunohistochemistry analysis of Ki-67 and S100A10 protein levels. Data were showed as mean ± standard deviation. Tumor volume at different time points was analyzed by repeated measures ANOVA, n = 4 for mice upon each treatment. * P < 0.05.

Article Snippet: After being blocked using 5% non-fat milk for 1 h at room temperature, the primary rabbit anti-human antibodies against S100A10 (ab76472, Abcam) and GAPDH (ab9485, Abcam) were supplemented overnight at 4°C.

Techniques: Knockdown, In Vivo, Transfection, Injection, Immunohistochemistry, Standard Deviation